Activity of Muntingia calabura Leaves Ethanolic Extract on Glucose and Insulin Blood Levels in Streptozotocin-induced Rat

  • Suci Syahara Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara.
  • Urip Harahap Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara.
  • Tri Widyawati Department of Pharmacology, Faculty of Medicine, Universitas Sumatera Utara.

Abstract

Objectives: The research  aims to determine the effect of Muntingia calabura leaves extract on improving diabetes conditions, by analyzing blood glucose and insulin levels of male Rattus novergicus which is given high fat diet and induce streptozotocin 30 mg/kg BB with a two-week interval.


Design: This research was a purely experimental with pretest and post test control group design. This research was divides into 6 groups. Three treatment groups were treated by Muntingia calabura leaves extract with the doses of 125, 250, and 500 mg/kg. Other groups are negative control (CMC-Na), positive control (glibenklamid) and normal control.


Interventions: The variables specified in this study are variations in the concentration of extract used. variations in extract concentration are expected to affect the results of glucose concentration and blood insulin in rats.


Main outcome measure: The main parameters measured in this study were rat blood glucose and insulin levels.


Results: The result of this research indicate that the ethanol extract of Muntingia calabura leaves able to reduce blood glucose levels effectively at dose 500 mg/kg (97, 17 mg/dL) so that insulin levels increase well (0,839 µg/mL).


Conclusion: Muntingia calabura leaves ethanol extract can reduce glucose levels and increase blood insulin levels in rats.


 

Keywords: Muntingia calabura, anti-hyperglycemia, insulin levels

Downloads

Download data is not yet available.

Author Biographies

Suci Syahara, Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara.

Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara.

Urip Harahap, Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara.

Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara.

Tri Widyawati, Department of Pharmacology, Faculty of Medicine, Universitas Sumatera Utara.

Department of Pharmacology, Faculty of Medicine, Universitas Sumatera Utara.

References

1. Krishnaveni M, Ravi D. Qualitative and Quantitative Study of Phytochemicals in Muntingia calabura L. Leaf and Fruit. World J. Pharm. Res. 2014; 3(6):1687–1696.
2. Buhian WPC, Rubio RO, Valle Jr, DL. and Martin-Puzon JJ. Bioactive metabolite profiles and antimicrobial activity of ethanolic extracts from Muntingia calabura L. leaves and stems. Asian Pac. J. Trop. Biomed. 2016; 6(8):682–685.
3. Mahmood ND. et al. Muntingia calabura: a review of its traditional uses, chemical properties, and pharmacological observations. Pharm. Biol. 2014; 52(12):1598–1623.
4. Sufian AS, Ramasamy K, Ahmat N, Zakaria ZA. & Yusof MIM. Isolation and identification of antibacterial and cytotoxic compounds from the leaves of Muntingia calabura L. J. Ethnopharmacol. 2013: 146(1):198–204.
5. Sridhar M, Thirupathi K, Chaitanya G, Kumar BR, Mohan GK. Antidiabetic Effect of Leaves of Muntingia calabura L., in Normal and Alloxan-Induced Diabetic Rats. Pharmacologyonline.2011; 2:626–632.
6. Sani MH, Zakaria ZA, Balan T, Teh LK. & Salleh M Z. Antinociceptive activity of methanol extract of Muntingia calabura leaves and the mechanisms of action involved. Evidence-Based Complement. Altern. Med. 2012; 1-10.
7. Herlina H, Amriani A, Solihah I & Sintya R. Antidiabetic Activity Test of Ethanolic Seri Leave’s (Muntingia Calabura L.) Extract in Male Rats Induced by Alloxan. Sci. Technol. Indones. 2018; 3(1):7–13.
8. Azwanida, N. N. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants; 2015; 4(3):3–8.
9. Zhang M, Lv XY, Li J, Xu ZG. & Chen L. The characterization of high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model. Exp. Diabetes Res. 2008; 1-9.
10. Yusoff N. et al. Nypa fruticans Wurmb. Vinegar’s Aqueous Extract Stimulates Insulin Secretion and Exerts Hepatoprotective Effect on STZ-Induced Diabetic Rats. Nutrients. 2017; 9(9):925.
11. Tripathy D. et al. Insulin secretion and insulin sensitivity in relation to glucose tolerance: lessons from the Botnia Study. Diabetes. 2000; 49(6):975–980.
12. Nijveldt RJ. et al. Flavonoids: a review of probable mechanisms of action and potential applications. Am. J. Clin. Nutr. 2001; 74(4):418–425.
13. Luzi, L. & Pozza, G. Glibenclamide: an old drug with a novel mechanism of action? Acta Diabetol. 1997; 34(4):239–244.
14. Sheppard DN. & Robinson KA. Mechanism of glibenclamide inhibition of cystic fibrosis transmembrane conductance regulator Cl− channels expressed in a murine cell line. J. Physiol. 1997; 503(2):333–346.
15. Kamalakkannan N. & Prince PSM Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin‐induced diabetic wistar rats. Basic Clin. Pharmacol. Toxicol. 2006. 98(1):97–103.
16. Coskun O, Kanter M, Korkmaz A. & Oter S. Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and β-cell damage in rat pancreas. Pharmacol. Res. 2005; 51(2):117–123.
17. Hii C S T & Howell SL. Effects of epicatechin on rat islets of Langerhans. Diabetes. 1984; 33(3):291–296.
18. Vessal M, Hemmati M & Vasei M. Antidiabetic effects of quercetin in streptozocin-induced diabetic rats. Comp. Biochem. Physiol. Part C Toxicol. Pharmacol. 2003; 135(3):357–364.
Statistics
243 Views | 186 Downloads
How to Cite
Syahara, S., Harahap, U., & Widyawati, T. ( ). Activity of Muntingia calabura Leaves Ethanolic Extract on Glucose and Insulin Blood Levels in Streptozotocin-induced Rat. Asian Journal of Pharmaceutical Research and Development, 7(4), 8-11. https://doi.org/https://doi.org/10.22270/ajprd.v7i4.552