Antimicrobial Activity of Ethanol Extract of Rhizome Turmeric (Curcuma Longa L.) For Growth of Escherichia coli, Staphylococcus aureus and Candida albicans

Authors

  • Gurning Kasta Department of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Medan, Indonesia.

DOI:

https://doi.org/10.22270/ajprd.v8i3.712

Keywords:

Extract, antimicrobial, Curcuma longa L, Escherichia coli, Staphylococcus aureus, Candida albicans

Abstract

Objective: The purpose of this study was to determine at what concentration of ethanol extract of rhizome turmeric is active against Escherichia coli, Staphylococcus aureus and Candida albicans.

Methods: Ethanolic extract of rhizome turmeric was tested for phytochemical screening by using standard protocol. Antimicrobial testing was using the diffusion disc method to measure the inhibition zone against the Escherichia coli, Staphylococcus aureus and Candida albicans with various concentration of rhizome turmeric extract (500 mg/mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 50 mg/mL, 25 mg/mL).

Results: Phytochemical screening showed that ethanolic extract of rhizome turmeric contain alkaloids, flavonoids, saponins, tannins, and triterpenoid/steroid. The antimicrobial inhibition of ethanol extract of rhizome turmeric against Escherichia coli, Staphylococcus aureus and Candida albicans microbia at a concentration of 500 mg/mL had a diameter of 15.7 mm, 15 mm and 15.18 mm with a strong category.

Conclusions: rhizome turmeric could be use as a novel antimicrobial agent.

 

 

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Author Biography

Gurning Kasta, Department of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Medan, Indonesia.

Department of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Medan, Indonesia.

References

1. Allawi SS, Jassim MA, Hiba QH, and Tagreed IA. The Effect of Curcuma longa (Turmeric) Rhizomes Extracts on Pathogenik Bacteria In Comparison With Standard Antibiotics. Journal of Biotechnology Research Center 2009; 3(1):15-20.
2. Rudrappa, T. And H.P. Bais. Curcumin A Know Phenolic From Curcuma longa Attenuates The Virulence of Pseudomonas aeruginosa PAO1 In Whole Plant and Animal Pathogenicity Models. J.Agric. Food Chem. 2008; 56:1955-1962.
3. Mohamed AM, FZ. El-Sharkawy, S.AA. Ahmed, WM. Aziz and O.A Badary. Glycemic Control and Therapeutic Effect of Nigella sativa and Curcuma longa on Rats With Streptozotocin Induced Diabetic Hepatopathy. J.Pharmacol.Toxicol. 2009; 4:45-57.
4. Villegas I, S. Sanshez-Fidalgo and LC. Alacon De La. New Mechanisms and Therapeutic Protential of Curcumin For Colorectal Cancer. Mol.Nutr.Food Res. 2008; 52:1040-1061.
5. Niamsa N and C. Sittiwet. Antimicrobial Activity of Curcuma longa Aqueous Extract. Journal Of Pharmacology and Toxycology. 2009; 1-5.
6. Chainani-Wu.N. Safety and Anti-inflammatory Activity of Curcumin: a component of turmeric (Curcuma longa). Journal of Alternative and Complemet Medicine.2003; 9:161-168.
7. Peter. K.V. Informatics on Turmeric and Ginger. India Spices 36 (2 and 3):12 -14.
8. Ikpeama, Ahamefula, Prof.Onwuka, G.I and Nwankwo, Chibuzo. Nutritional Composition of Turmeric (Curcuma longa) And Its Antimicrobial Properties. International Journal of Scientific & Engineering Research.2014; 5(10):1085-1089.
9. Kaper JB, James PN., and Harry LT Mobley. Pathogenic Escherichia coli. Nature Reviews Microbiology. 2004; 2:123-140.
10. Tong SYC, Davis JS, Eichenberger E, Holland TL, Jr VGF. Staphylococcus aureus Infections: Epidemiology, Pathophysiology. Clinical Microbiology Reviews 2015; 28(3):602-31.
11. Moyes DL and Julian RN. Review Article Mucosal Immunity and Candida albicans Infection. Hindawi Publishing Corporatin Clinical and Developmental Immunology. 2011;1-9.
12. Sinaga SM, Haro G, Sudarmi S, Iksen I. Phytochemical Screening and Antihyperglycemic Activity of Ethanolic Extract of Coriandrum sativum L. Leaf 2019; 12(4):1992-6.
13. Simanjuntak, HA. Antibacterial Activity of Ethanolic Extract of Kitolod (Hippobroma longiflora) Leaf Against Staphylococcus aureus and Salmonella typhi. Asian Journal of Pharmaceutical Research and Development. 2020; 8(1):52-54.
14. Davis WW, Stout TR. Disc Plate Method of Microbiological Antibiotic Assay: Applied and Enviromental Microbiology 2009; 22(4):666-70.
15. Olajuyigbe OO. Synergistic Influence of Tetracycline on the Antibacterial Activities of Amoxicillin Against Resistant Bacteria. Journal of Pharmacy and Allied Health Sciences. 2012; 2(1):12-20.
16. Sobel JD and N.Obedeanu. Effects of Subinhibitory Concentrations of Ketoconazole on in Vitro Adherence of Candida albicans to Vaginal Epithelial Cell. Eur.J.Clin.Microbiol.1983; 2(5):445-452.
17. Karou D, Savadogo A, CaniniA,Yameogo S, Montesano C, Simpore J, Colizzi V, Traore AS. Antibacterial activity of alkaloids from Sidaacuta 2005; 4(12):1452-7.
18. Dzoyem JP, Hiroshi H, Barthelemy N, Bonaventure TN, Kazuhisa S. Antimicrobial Action Mechanism of Flavonoids From Dorstenia Spesies. Drug Discoveries & Therapeutic 2013; 7(2):66–72.
19. Wu T, Mengying H, Xixi Z, Ying Z, Tianfu Q, Siyi P, Xiaoyun X. A StructureActivity Relationship Study of Flavonoids As Inhibitors of E. coli By Membrane Interaction Effect. Biochimica et Biophysica Acta2013; 2013:2751–6.
20. Khan MI, Abdulatef A, Jin HS, Jun SB, Min YK,Jong DK. Green Tea Seed Isolated Saponins Exerts Antibacterial Effects Against Various Strains of Gram Positive and Gram Negative Bacteria A Comprehensive Study In Vitro and In Vivo.Evidence-Based Complementary and Alternative Medicine2018;1–12.
21. Prasad, N.R., Viswanathan S., Devi, J.R., Nayak V. Swetha, C.V. Archana, B.R., Parathasarathy, N. and Rajkumar J. Preliminary Phytochemical Screening and Antimicrobial Activity of Samanea saman. Journal of Medicinal Plants Reseacrh. 2008; 2(10):268 – 270.
22. Dharmananda, S. Gallnut and The Uses of Tannins in Chinese Medicine, In: Proceedings of Institute For Traditional Medicine. Portland. Oregon.
23. Haraguchi, H., Shintaro K., Shiho O., Muhammad H and Kozo Shibata. 1999. Antimicrobial Triterpenes From Illex integra and The Mechanisme of Antifungal Action. Phytotherapy Research. 1999; 13:151 – 156.

Published

2020-06-15

How to Cite

Kasta, G. (2020). Antimicrobial Activity of Ethanol Extract of Rhizome Turmeric (Curcuma Longa L.) For Growth of Escherichia coli, Staphylococcus aureus and Candida albicans. Asian Journal of Pharmaceutical Research and Development, 8(3), 5–8. https://doi.org/10.22270/ajprd.v8i3.712