Test of Active Compound and Activity Pirdot Leaf Extract (Sauraia Vulcani Korth.) on Candida Albicans Growth in Vitro
DOI:
https://doi.org/10.22270/ajprd.v8i5.825Keywords:
Antifungal; Candida albicans; Diffusison Method; Saurauia vulcani KorthAbstract
Objective: to determine the activity of pirdot leaf extract on the growth of Candida albicans in vitro
Design: this study was a true experiment with a post-test only control group research design. The method used agar diffusion using Kirby Bauer technique. This study used 4 variations concentration namely 10%, 20%, 40%, and 80%, positive control (ketoconazole tablets), and negative control (Dimethyl Sulfoxide). The data obtained were analyzed using descriptive statistical analysis.
Interventions: the intervened variable were compound content and antifungal activity of pirdot leaf extract concentration of inhibitory effect.
Main outcome measures: the main measurement in this study was the inhibition concentration of pirdot leaf extract on Candida albicans growth
Results: the results showed that the inhibitory from a concentration of 10% of 8.96 mm, the highest inhibition was at 80% of 18.33 mm.
Conclusion: it could be concluded that pirdot leaf extract has antifungal activity against Candida albicans.
Keywords: Antifungal; Candida albicans; Diffusison Method; Saurauia vulcani Korth
Downloads
References
2. Sherrard J, Donders G, White D, Jensen JS (2011) European (IUSTI/WHO) guideline on the management of vaginal discharge. International journal of STD & AIDS. 22(8), pp 421-9. https://doi.org/10.1258/ijsa.2011.011012
3. Katzung, BG, Masters SB, Trevor, AJ (2014) Farmakologi Dasar & Klinik. Vol.2, Edisi 12, Editor Bahasa Indonesia Ricky Soeharsono et al. Jakarta, Kedokteran EGC.
4. Fagbohun ED, Lawal OU, Ore Me (2012) The Antifungal Activities of the Methanolic Crude Extract of the Leaves of Ocimum gratissimum L., Melanthera scandens A. and Leea guineensis L. on some Phytapogenic Fungi. International Jounal of Biology, vol 1, pp 12-21.
5. Muhlisah F (2005) Tanaman Obat Keluarga. Jakarta, Penebar Swadaya.
6. Dalimartha S (2006) Atlas Tumbuhan Obat Indonesia Jilid 4, Jakarta, Puspa Swara.
7. Sati SC, Joshi S (2011) Aspects of Antifungal Potentialof Ethnobotanically Known Medicinal Plants. Research Journal of Medicinal Plant. Vol 5(4), pp 377-91. http://dx.doi.org/10.3923/rjmp.2011.377.391
8. Arif T, Bhosale JD, Kumar N, Mandal TK, Bendre RS, Lavekar GS, et al (2009) Natural Products-Antifungal Agents Derived From Plants. Journal of Asian Natural Products Researc. Vol 11(7), pp 621-38. https://doi.org/10.1080/10286020902942350
9. Lippold LE, Draeger T, Teichert A, Wessjohann L, Westermann B, Rosahl S, et all (2009) Antioomycete Activity Of Gamma-Oxocrotonate Fatty Acids Against Phytophthora infestans. Journal Agricultural and Food Chemical. Vol 57(20), pp 9607-12. https://doi.org/10.1021/jf902067k
10. Roking (2007) Identifikasi Golongan Senyawa Dan Aktivitas Antioksidan Ekstrak Metanol, Serta Fraksi Aktif Daun Pirdot (Saurauia vulcani Korth.),[skripsi]. Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia.
11. Marbun, R. Siregar, S., Hasibuan, A., Sinurat, J., Syarifuddin, A., Octora, D, Rizky, V. and Gurusinga, R (2019) The Immunomodulatory Activity of Pirdot Leaf Extract (Sauraia Vulcani korth.) on the Immune System of Male Rats. In Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT, ISBN.
12. Mukherjee PK, Chandra J, Kuhn DM, Ghannoum MA (2003) Mechanism of fluconazole resistance in Candida albicans biofilms: phase-specific role of efflux pumps and membrane sterols. Infection and immunity. Vol 71(8): pp 4333-40.https://dx.doi.org/10.1128%2FIAI.71.8.43334340.2003
13. Al-Kobaisi MF. Jawetz, Melnick & Adelbergs (2007) Medical Microbiology 24th Edition. Sultan Qaboos University Medical Journal [SQUMJ]. Vol 7(3), pp 273-5.
14. Harborne JB (1998) Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Bandung: Institut Teknologi Bandung.
15. Ditjen POM (1995) Farmakope Indonesia edisi IV. Jakarta, Depkes RI.
16. Turnip CD (2014) Uji Daya Hambat Ekstrak Umbi Pakupohon (Cyathea contaminans (Hook.) Copel) Terhadap Jamur Microsporum gypseum Secara In Vitro [skripsi]. Medan, Fakultas Pertanian Universitas Sumatera Utara.
17. Alfiah RR, Khotimah S, Turnip M (2015) Efektivitas ekstrak metanol daun sembung rambat (Mikania micrantha kunth) terhadap pertumbuhan jamur Candida albicans. Protobiont. Vol 4(1), pp 52-7. https://jurnal.untan.ac.id/index.php/jprb/article/view/8735/8710
18. De Ornay AK., Prehananto H, Dewi ASS (2017) Daya Hambat Pertumbuhan Candida albicans dan Daya Bunuh Candida albicans Ekstrak Daun Kemangi (Ocimum sanctum L.), Jurnal Wiyata. Vol 4(1), pp 78-83.
19. Mulangsri DA, Nurani LH (2014) Aktivitas Antifungi Fraksi Etil Asetat Ekstrak Daun Pacar Kuku Terhadap Candida albicans Resisten Flukonazol. Media Farmasi. Vol 12(1): pp 45-56. http://dx.doi.org/10.12928/mf.v12i1.3017
20. Masloman AP (2016) Uji Daya Hambat Ekstrak Daun Sirsak (Annona murcata L.) Terhadap Pertumbuhan Jamur Candida Albicans. Pharmacon. Vol 5(4). https://doi.org/10.35799/pha.5.2016.13975
21. Quintin J, Saeed S, Martens JH, Giamarellos-Bourboulis EJ, Ifrim DC, Logie C, et al (2012) Candida albicans infection affords protection against reinfection via functional reprogramming of monocytes. Cell host microbe. Vol 12(2): pp 223-32.https://doi.org/10.1016/j.chom.2012.06.006
22. Bhaskara GY (2012) Uji daya antifungi ekstrak etanol daun salam (Syzygium polianthum) terhadap Candida albicans secara in vitro [skripsi]. Surakarta, Fakultas Kedokteran Universitas Muhammadiyah Surakarta.
23. Febriani TH (2014) Uji Daya Antifungi Jus Buah Pare (Momordica charantia L) Terhadap Daya Hambat Pertumbuhan Candida Albicans Secara in vitro. Skripsi. Surakarta, Fakultas Kedokteran Gigi Universitas Muhammadiyah Surakarta.
24. Octora, D., Teresia Marbun, R., & Koto, R. (2019). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Pirdot (Saurauia Vulcani korth.) Terhadap bakteri Salmonella thypi. Jurnal farmasimed (JFM), 2(1), 40-44. https://doi.org/10.35451/jfm.v2i1.286
25. Sudbery PE (2011) Growth of Candida albicans hyphae. Nature Reviews Microbiology. Vol 9 (10): pp 737-
Published
How to Cite
Issue
Section
AUTHORS WHO PUBLISH WITH THIS JOURNAL AGREE TO THE FOLLOWING TERMS:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).