Antifungal Active Fraction of Peria Pantai(Colubrina Asiatica (L.) Brong) Leaf Against Fluconazole-Resistance Candida Albicans
DOI:
https://doi.org/10.22270/ajprd.v9i2.936Keywords:
Colubrina asiatica, Antifungal agent, Fluconazole-Resiatance Candida albicans, Kirby-Bauer method, secondary metabolite.Abstract
Objective: A novel antifungal needs to be found to overcome fungal resistance to antifungal drugs. Medicinal plants contain biologically active compounds as antifungal agents. Peria Pantai (Colubrinaasiatica L. Brong) has been used as a folk medicine by the Acehnese community to treat skin disease. This research aim was to evaluate antifungal activity of C. asiatica leaf fraction against Candidaalbicans resistant fluconazole.Methode: The extraction was accomplished consecutively as solvent multistep using n-hexane, ethyl acetate, and methanol. Phytochemical investigation has done to each extract/fraction. The antifungal activity has been evaluated by Kirby-Bauer method.Result: The result of phytochemical investigation of n-hexane fraction contains steroid compound, ethyl acetate fraction contains saponin and steroid compounds, and the methanol fraction contains flavonoid, saponin, tannin, and steroid compounds. n-Hexane and ethyl acetate fraction of Peria Pantai leaf have not shown antifungal activity, while it has been shown on methanol fraction with inhibition zone 5,6; 7,17; 8,25; and 11,4 mm at 10%, 20%, 30% and 40%concentration.
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2. Al Aboody MS, Mickymaray S. AntiFungal Efficacy and Mechanisms of Flavonoids. Antibiotics (Basel). 2020; 9(2):45.
3. Center for Disease Control and Prevention (CDC). Antibiotic Resistance Threats in the United States 2013. U.S Departement of Health and Human Services.
4. Desai N, and Gaikwad DK. EssentialOil Composition And Antibacterial Activity of Colubrina asiatica (L.) Brongn. Asian Journal of Pharmaceutical Research and Development. 2014; 2(4):13-17.
5. Evans CW. Pharmacognosy Trease and Evans 16th Edition. London: Saunders Elsevier; 2009. p.25-30
6. Harborne JB. Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. London, 2rd ed. New York: Springer Science & Business Media; 2012. p.4-16
7. Johnston MC. Revision of Colubrina (Rhamnaceae). Brittonia. 1971; 23:2-53.
8. Kumar RB, Suryanarayana B. Antimicrobial Screening of Some Selected Tribal Medicinal Plants From Sriharikota Island. Andhra Pradesh,India; Etnobotanical Leaflets. 2008; 12:1269-82
9. Lee SS, Chen WC, Chen CH. New Jujubogenin Glycosides From Colubrina asiatica. J. Nad. Prad. 2000; 63:1580-1583
10. McCormick CM. Colubrina asiatica (LatherLeaf). Management Plant Center for Aquatic and Invasive Plants. University of Florida,Florida. 2007.
11. Nandwani D. Calvo JA. Tenorio J. Calvo F. Manglona L. Medicinal Plants and Traditional Knowledge in the Northern Mariana Islands. Journal of Applied Biosciences. 2008; (2):323– 330.
12. Ribera AE, Zuniga G. Induced plant secondary metabolites for phytopatogenic fungi control: a review, Journal of Soil Science and Plant Nutrition. 2012;12(4), 893-911
13. Sangsopha W, Kanokmedhakul K, Lekphrom R.,et all. Chemical constituents and biological activities from branches of Colubrina asiatica . Natural Product Research. 2018; 32(10)
14. Thaman,R.R., and Batiri,K.B.,.The Ethnobotany of Pacific Island Coastal Plants. Atoll Research Bulletin. 1992; 1:361
15. Wagner H, Ott S et al. Chemistry, 13C-NMR Study and Pharmacology of Two Saponins from Colubrina asiatica. Planta Med. 1983; 48(7):136-41.
16. Whaley SG, Berkow EL, Rybak JM, Nashimoto AT, Barker KS, Rogers PD. Azole Antifungal Resistance in Candida albicans and Emerging Non-albicans Candida Species. Front Microbiol. 2016; 7:2173
17. World Health Organization (WHO). Quality Control Methods for Medicinal Plant Materials. WHO. 2012. p.29-33.
18. World Health Organization (WHO). WHO Laboratory Manual for Diagnosis of Fungal Opportuistic Infections in HIV/AIDS Patients. 2009.
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