Vigna Comosa Baker Aerial Part’s Extracts Effects on Hyperglycemia and Hepatic Glucose’sliberation
DOI:
https://doi.org/10.22270/ajprd.v9i2.965Keywords:
Vignacomosa, extracts, phytochemistry,cytotoxicity,hyperglycemia, hepatic glucose.Abstract
Vignacomosa Baker (V. Comosa) is a plant used by Beninese Oueme department’s populationfor the treatment of diabetes without any scientific study showing its effectiveness. The objective of this study is to investigate the effects of theextracts of V. Comosaon hyperglycemic rabbits by Oral Glucose Tolerance Test (OGTT) and on hepatic glucose liberation. Phytochemical screening revealed that the plant contains alkaloids, tannins, flavonoids, mucilage, triterpenoids, steroids, reducing compounds, saponins, oses and holosides.Cytotoxity test showed that those extracts were free of toxicity. The extracts showed anti-hyperglycemic activities doses and time depending. The effective doses are 500 mg/kg for the aqueous extract and 1000 mg/kg for the ethanolic extract. The extracts are effective as compared with glibenclamide (reference product). Moreover, the ex vivo test conducted on the liver revealed that V. Comosa aqueous extract inhibits the hepatic glucose liberation and 500 mg/mL is the most effective dose. The results of this study account for the plant under consideration of its traditional usage in the treatment of diabetes.
Downloads
References
2. modèle expérimental de diabètealloxanique. Phytothérapie. 2011; (9):274-282.
3. Shaw J, Sicree R,Z immet P. Global estimates of the prevalence of diabetes for 2010 and 2030. DiabetesRes. Clin. Pract.2010; 87(1): 4-14.
4. Gning SB, Thiam M, Fall F, Ba-Fall K, Mbaye PS, Fourcade L. Le diabètesucré en Afriquesubsaharienne: aspects épidémiologiques, difficultés de prise en charge. Med Trop. 2007; 67:607-611.
5. Djrolo F, Gbary A, Houinato D, Fambo D, Djigbénoudé O. Prevalence of diabetes mellitus and associated factors in the general population in Benin. world diabetes congress, Abstract books, 2011, 570.
6. Organisation mondiale de la Santé – Profils des pays pour le diabète, 2016.
7. Famuyiwa OO. The efficacity of traditional medicine in the management of diabetes mellitus in southwestern Nigeria. Afr J Med Sci. 1993; 22: 31-37.
8. Houghton PJ, Amala R. Laboratory Handbook for the Fractionation of Natural Extract Pharmacognosy Research Laboratries, Departement of pharmacy, king's College, London. 2009; 212 p
9. Michael AS, Thompson CG, Abramovitz M. Artemia Salina as a test organism for a bioassay. Sci. 1956; 123:467-505.
10. Lawson-Evi P, Eklu-Gadégbéku K, Aklikokou AK, Gbéassor M. Activitéhypoglycémiante de quelquesplantesmédicinales. Pharm Med Trad Afr. 1997; (9): 60-69.
11. Agbodjogbé WK, Dosseh K, Gbenou DJ, Dansou HP. Etude des propriétés des activités antihyperglycémiantesd' extraits du mélange de feuilles-écorces du Casuarinae quisetifolia (Filao): étude in vivo sur le lapin albinos. Re. Ivoir. SciTechnol. 2015; 25: 162-176.
12. Agbodjogbé W, Dosseh K, Gaturagi C, Ahounou J, Kpatcha T, Kerekou A.C, et al. Evaluation des activités antihyper glycémiantesd’extraitsaqueux de l’écorce et des feuilles du Casuarinaequisetifolialinn.J Rechsci Uni. Lomé (Togo), 2015; 17(1): 171-181.
13. Houndjo FC, Agbodjogbe W, Assogba F M, Kohoude J M, Ayedoun M A, Dansou PH et al. Comparative study of Antihyperglycemic activity of aqueous extracts from the leaves of Brideliaferruginea, Lophiralanceolata and Oxytenantheraa by ssinica, with their mixture. Int. J. Curr. Res. Chem. Pharm. Sci. 2017; 4(11):22-33.
14. Akakpo B H, Akpovi C D, Medehouenou TCM, Assogba MF, Dansou PH, Gbenou JD. Conyzaaegyptiaca (L.) Dryand ex. Aiton extracts exhibite antioxidant activity and prevent hepatic glucose liberation in vitro. Int J of Biosci. 2016; 9:431-439.
15. Sakine MNA, Mahmout Y, Dijoux-franca MG, Gbenou J, Moudachirou M. In vitro anti-hyperglyceamic effect of glucocapparin isolated from the seeds of Bosciasenegalensis (Pers.) Lam.exPoiret. African Journal of Biotechnology. 2012; 11(23), 6345-6349.
16. Takin M, AHOKPE M, ZOHOUN L, BABA-MOUSSA L, SEZAN A. Effect of total khayasenegalensis (Meliaceae) barks extracts on hepatic liberation of glucose. National Journal of Physiology, Pharmacy & Pharmacology. 2014, 4 (2): 105-10.
17. Bruneton J. Pharmacognosie, phytochimie - Plantesmédicinales - Techniques et documentations. 4ème Edition. Paris; 2009; p 1167-1245.
18. Jose Luis C, Zaira L, Hernandez I, Pilar P, Maria D, Garcia G. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products. BMC Biotechnology. 2002; 2:17-35.
19. Mousseux M. Test de toxicitésurlarvesd' Artemiasalina: entretien d'un élevage de balanes. Nouméa: Centre Universitaire de Nouvelle Calédonie, 1995; 20 p
20. Akakpo BH, Akpovi DC, AhounouAïkpé FJ, Kinsou CDL, Gbénou DJ, Dansou HP.Conyzaaegyptiaca(L.) Dryand ex. Aiton effect on blood glucose levels in normal albinos rats through oral glucose tolerance test. Int. J. Curr. Res. Chem. Pharm. Sci. 2017; 4(4): 19-26.
21. Amadou A. Etude d’unerecettetra ditionnelle, des écorces de tronc de Sclerocaryabirrea Hosch et de Uapacatogoensis Paxutilisées dans le traitement du diabète. [Thèse]. Pharmacie: Bamako; 2006; 107p.
22. Sreerama YN, Takahashi Y, Yamaki K. Phenolic antioxidants in some Vigna species of legumes and their distinct inhibitory effects on α-glucosidase and pancreatic lipase activities. J Food sci. 2012; 77(9):C927-33.
23. Yeo JY, Ha TJ, Nam JS, Jung MH. Antidiabetic effects of Vignanakashimae extract in db/db mice. BiosciBiotechnolBiochem.2011; 75(11):2223-8
24. Yao Y1, Cheng X, Wang S, Wang L, Ren G. Influence of altitudinal variation on the antioxidant and antidiabetic potential of azuki bean (Vignaangularis).Int J Food Sci Nutr. 2012; 63(1):117-24.
25. Itoh T1, Kobayashi M, Horio F, Furuichi Y. Hypoglycemic effect of hot-water extract of adzuki (Vignaangularis) in spontaneously diabetic KK-A(y) mice. Nutrition. 2009 25(2):134-41.
26. Assogba MF. Phytochimie et proprieties pharmaco biologiquesd' extraits des feuilles de Eleaisguineensis Jacq (arecaceae). [Thèse]. Abomey-Calavi: Universitéd' Abomey-Calavi; 2016.
Published
How to Cite
Issue
Section
AUTHORS WHO PUBLISH WITH THIS JOURNAL AGREE TO THE FOLLOWING TERMS:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).