Analytical Method Development and Validation of Brivaracetam in API and Marketed Formulation by RP-HPLC

Authors

  • Banothu Bhadru CMR College of Pharmacy, Kandlakoya, Medchal, Hyderabad, Telangana, India.
  • Peddinti Naveen Kumar Research Scholar, JSS College of Pharmacy, Mysuru, Karnataka, India.
  • Damera Sujatha University College of Pharmaceutical Sciences, Kakatiya University, Hanamakonda, Warangal, Telangana, India.
  • Pranay Renukuntla School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Medchal-Malkajgiri, Hyderabad, Telangana, India.
  • Narender Boggula CMR College of Pharmacy, Kandlakoya, Medchal, Hyderabad, Telangana, India.

DOI:

https://doi.org/10.22270/ajprd.v12i2.1372

Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of brivaracetam in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry ODS RP C18,5mm, 15mm x 4.6mm column using a mixture of phosphate buffer: methanol: acetonitrile (30:35:35% v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 285 nm. The retention time of the brivaracetam was 2.183. The method produces linear responses in the concentration range of 60-140μg/ml of brivaracetam. The method precision for the determination of assay was below 2.0%RSD. The method was found to be sensitive, accurate and precise useful in the quality control of bulk and pharmaceutical formulations.

 

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Author Biographies

Banothu Bhadru, CMR College of Pharmacy, Kandlakoya, Medchal, Hyderabad, Telangana, India.

CMR College of Pharmacy, Kandlakoya, Medchal, Hyderabad, Telangana, India.

Peddinti Naveen Kumar, Research Scholar, JSS College of Pharmacy, Mysuru, Karnataka, India.

Research Scholar, JSS College of Pharmacy, Mysuru, Karnataka, India.

Damera Sujatha, University College of Pharmaceutical Sciences, Kakatiya University, Hanamakonda, Warangal, Telangana, India.

University College of Pharmaceutical Sciences, Kakatiya University, Hanamakonda, Warangal, Telangana India.

Pranay Renukuntla, School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Medchal-Malkajgiri, Hyderabad, Telangana, India.

School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Medchal-Malkajgiri, Hyderabad, Telangana, India.

Narender Boggula, CMR College of Pharmacy, Kandlakoya, Medchal, Hyderabad, Telangana, India.

CMR College of Pharmacy, Kandlakoya, Medchal, Hyderabad, Telangana, India.

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Published

2024-04-15 — Updated on 2024-04-16

Versions

How to Cite

Bhadru, B., Naveen Kumar, P., Sujatha, D., Renukuntla, P., & Boggula, N. (2024). Analytical Method Development and Validation of Brivaracetam in API and Marketed Formulation by RP-HPLC. Asian Journal of Pharmaceutical Research and Development, 12(2), 154–159. https://doi.org/10.22270/ajprd.v12i2.1372 (Original work published April 15, 2024)

Issue

Vol. 12 No. 2 (2024): Vol 12 No 2 (2024): Volume 12 Issue 2 March. - April. 2024

Section

Research Articles

Copyright (c) 2024 Banothu Bhadru, Peddinti Naveen Kumar, Damera Sujatha, Pranay Renukuntla, Narender Bogula

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