In Vivo Antimicrobial Activity Of Indian Medicinal Plant Tinospora Cordifolia Using Serial Tube Dilution Technique

Authors

  • Abhimanyu Sharma Department of Botany, University of Rajasthan, Jaipur. Rajasthan, India
  • A Batra Department of Botany, University of Rajasthan, Jaipur. Rajasthan, India

Keywords:

Tinospora Cordifolia, Plant extracts, Antibacterial, MIC, Antimicrobial activity

Abstract

The present study designed for the methanolic extracts of different in vivo (root, stem, leaf and bark) plant samples of Tinospora cardifolia (family: Menispermaceae) were screened for their antimicrobial activity against Escherichia coli, E. cloacae, Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, A. niger, A. flavus, A. solani, R. stolonifer and F. oxisporum by using the MIC “Serial tube dilution technique” and agar well diffusion method. It is clear from the results that, the extracts of these plants acts as a good source of antibiotics against various bacterial and fungal pathogens tested and exhibited broad spectrum of antimicrobial activity. The largest zone of inhibition was observed for methanolic leaf extract against Staphylococcus aureus (15.5 ± 0.14 mm) and minimum was observed in bark against F. oxisporum and Pseudomonas aeruginosa (6.0 ± 0.45mm). Minimum inhibitory concentration (MIC) may be defined, as the lowest concentration of antimicrobial agent requires to inhibit the growth of organism. The maximum MIC values were recorded in stem and leaf i.e 128 µg/ml against Bacillus cereus, Staphylococcus aureus, A. niger, A. flavus, A. solani and R. stolonifer.The study suggest further research regarding the pharmacological investigations of this plant and also support the continued sustainable use of these plants in traditional systems of medicine.

Downloads

Download data is not yet available.

Published

2013-03-01

How to Cite

Sharma, A., & Batra, A. (2013). In Vivo Antimicrobial Activity Of Indian Medicinal Plant Tinospora Cordifolia Using Serial Tube Dilution Technique. Asian Journal of Pharmaceutical Research and Development, 1(2), 148–156. Retrieved from https://www.ajprd.com/index.php/journal/article/view/60

Issue

Volume 1st, Issue 2nd, Mar-Apr 2013

Section

Research Articles

AUTHORS WHO PUBLISH WITH THIS JOURNAL AGREE TO THE FOLLOWING TERMS:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).