Anti-Inflammation Activity of Virgin Coconut Oil In- Vitro Against Raw Cells 264.7
DOI:
https://doi.org/10.22270/ajprd.v8i1.633Keywords:
VCO, Antiinflammation, RAW 264.7 Cells, Gene Expression, LipopolysaccharidesAbstract
Objective:This study aimsto determine the anti-inflammation activity of pure coconut oil on Raw Cell 264.7 induced by LPSMethods: The anti-inflammation activity test for Virgin Coconut Oil (VCO) was carried out by testing the expression of TNF-α, IL-6, IL-1β, iNOS, and COX-2 genes on RAW 264.7 cells induced by LPS by the method Reverse Transcription-Polymerase Chain Reaction (RT-PCR).Results: The test results of iNOS, TNF-α, IL-6, IL-1β, and COX-2 gene expression from VCO on RAW 264.7 cells induced by lipopolysaccharide (LPS) decreased the value of VCO density. In iNOS expression, VCO density values (0,90±0,015) showed significantly different effects with positive and negative controls p<0,05, VCO IL-1β density values (2,47±0,010) showed different effects. significant with normal control, positive control and negative control p<0,05, TNF-α density value on VCO (0,91±0,010) showed significantly different effects from positive control and negative control p<0,05, then the value IL-6 density at VCO (1,23 ± 0,015) showed significantly different effects from normal and positive controls p<0,05, and COX-2 density values at VCO (1,02±0,015) showed significantly different effects with normal and positive controls p<0,05.Conclusion: Based on the results of this study VCO on RAW 264.7 cells induced by LPS can inhibit the expression of TNF-α, IL-6, IL-1β, iNOS, and COX-2 genes so that VCO effectively has anti-inflammatory activity.
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2. Chung, H.Y., Kim, H.J., Kim, K.W., Choi, J.S. and Yu BP. Molecular inflammation hypothesis of aging based on the anti-aging mechanism of calorie restriction. Microsc Res Tech.2002; 59: 264 272.
3. Dayrit, F.M., Buenafe, O.E.M., Chainani, E.T., de Vera, I.M.S., Dimzon, I.K.D., Gonzales, E.G., Santos, J.E.R. et al (2007). Standards for essential composition and quality factors of commercial virgin coconut oil and its differentiation from RBD [refined bleached and deodorized]. Philipp. J. Sci. Philipp.
4. Laveti, D., Kumar, M., Hemalatha, R., Sistla, R., Naidu, V. G., Talla, V., Nagpal, R., et al. Anti-inflammatory Treatments For Chronic Diseases: A review. Inflammation & Allergy Drug Targets, 2013; 12(5).349–361.
5. Man, Y.B.C. dan Manaf, M.A. Medium-Chain Triacylglycerols. In book Nutraceutical and Specialty Lipids and their Co-Products. Editor: Shahidi, F. USA: Taylor & Francis Group, LLC. Halaman: 2006; 31-32.
6. Novycha. A., Rosidah.,Yuandani., Suryani. S., and Satria.D.The Immunomodulatory Activities of PicriaFel-TerraeLour Herbs towards RAW 264.7 Cells.Journal of Medical Science. 2019; 7(1): 24-28.
7. Hadiarto, T., Listanto, E., Eny, I. and Riyanti. Identification of RB gene cDNA in Genetically Modified Potato Katahdin SP951.Jurnal AgroBiogen. 2015; 11(2): 59-64.
8. Silalahi, J and Surbakti, C. Burn Wound Healing Activity of Hydrolyzed Virgin Coconut Oil. USA. IJPRIF.2015; 8(1):67-73.
9. Sumiwi, A.S., Ramadhani, N. (2015). Antiinflammatory Activity of Various Plants Allegedly Derived from Flavonoids. Padjadjaran University Faculty of Pharmacy.Farmaka 2015; 14(2):112.
10. Yu, G. J., Choi, I. W., Kim, G. Y., Kim, B. W., Park, C., Hong, S. H., et al. Anti-inflammatory potential of saponins derived from cultured wild ginseng roots in lipopolysaccharide-stimulated RAW 264.7 macrophages. International journal of molecular medicine, 2015; 35(6): 1690-1698.
11. Kim, G.T., Tran, N.K.S., Choi, E.H., Song, Y.J., Song, J.H, Shim, S.M. et al. Immunomodulatory Efficacy of Standardized Annonamuricata (Graviola) Leaf Extract via Activation of Mitogen-Activated Protein Kinase Pathways in RAW 264.7 Macrophages.Evidence-Based Complementary and Alternative Medicine:2016; 1-10.
12. Lawrence, T. and Fong, C. (2010). The Resolution of Inflammation: Anti-Inflammatory Roles for NF kappa B. The International Journal of Biochem-istry & Cell Biology, 2010; 42(4), 519–523.
13. Yanti., Pramudito., E.T., Nuriasari. N. and Juliana.K. (2011).Lemon Pepper Fruit Extract (Zanthoxylumacanthopodium DC.) Suppresses the Expression of Inflammatory Mediators in Lipopolysaccharide-Induced Macrophages In Vitro.American Journal of Biochemistry and Biotechnology.2010; 7(4):190-195.
14. Kim, G.T., Tran, N.K.S., Choi, E.H., Song, Y.J., Song, J.H, Shim, S.M. et al. Immunomodulatory Efficacy of Standardized Annonamuricata(Graviola) Leaf Extract via Activation of Mitogen-Activated Protein Kinase Pathways in RAW 264.7 Macrophages.Evidence-Based Complementary and Alternative Medicine: 2016; 1-10.
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