HPLC & It’s Utilization in Disease Diagnosis
DOI:
https://doi.org/10.22270/ajprd.v8i2.666Keywords:
Hemoglobinopathies, Thalassemias, Glycosylated, Resolution, ClinicohematologicalAbstract
High Performance Liquid Chromatography is a technique in analytical chemistry used to separate, identify and quantify each component in a mixture. The HPLC is suitable for a variety of clinical applications, including pharmaceutical development, legal application such as detecting the Presence of illicit drugs in urine & blood. High-performance liquid chromatography is a technique introduced for the accurate diagnosis of hemoglobinopathies, thalassemias & estimation of glycosylated hemoglobin. The advantage of the HPLC system is the excellent resolution, reproducibility & quantification of several normal & abnormal hemoglobin resulting in accurate diagnosis of thalassemia syndromes. HPLC technique which is used to separate the component in across the two equivalents phases i.e. stationary phases is solid or liuqid supported on a solid which is packed into a column while sample moves in along with mobile phase is liuqid or gas. The purpose of this study is to evaluate the HPLC technique in diagnosis of thalassemia syndromes and also correlate it with clinicohematological profile in these cases.
Downloads
References
2. Schneider RG, Alperin JB, Lehmann H. Sickling tests. Pitfalls in Performance and interpretation.JAMA.1967;202(5):419–421.doi: 10.1001/jama.1967.03130180085017.
3. Wilson JB, Headlee ME, Huisman THJ. A new high-performance liquid chromatographic procedure for the separation and quantitation of various hemoglobin variants in adults and newborn babies. J Lab Clin Med 1983;102:174-185.
4. Rogers BB, Wessels RA, Ou CN, Buffone GJ. High-performance liquid chromatography in the diagnosis of hemoglobinopathies and thalassemias. Am J Clin Pathol 1985;84:671-674.
5. kutlar A, Kutlar F, Wilson JB, Headlee ME, Huisman THJ. Quantitation of hemoglobin components by high-performance cation-exchange liquid chromatography: its use in diagnosis and in the assessment of cellular distribution of hemoglobin variants. Am J Hematol 1984;17:39-53.
6. Ou CN, Rognerud CL. Rapid analysis of hemoglobin variants by cation-exchange HPLC. Clin Chem 1993;39:820-824.
7. Wajcman H, Bardakdjian J, Ducrocq R. Structural characterization of abnormal hemoglobins from dried blood specimens in a neonatal screening program. Ann Biol Clin1993;50:867-870.
8. OU C-N, Rogerud CL. Diagnosis of Haemoglobinopathies: Electrophoresis vs. HPLC. Clin Chim Acta 2001, 313:187-94.
9. Waters HM, Howarth JE, Hyde K, Goldstone S, Cinkotai KI, Richards JT. An evaluation of the Bio-Rad Variant Hemoglobin Testing System for the detection of haemoglobinopathies. Clin Lab Haematol 1998;20:31-40.
10. Kinberger B. Holmen A. Simultaneous determination of acetaminophen, theophylline and salicylate in serum by high-performance liquid Analytical reviews in clinical chemistry 563 chromatography. J Chromatogr 1982; 229: 492-7.
11. M. S. Tswett, On the new form of adsorption phenomena and its application in biochemical analysis, Proc. Warsaw Natural Biol. Soc. 1903, 14, 20.
12. Giordino PC In: Diagnostics and Prevention of Hemoglobinopathies using High Performance Liquid Chromatography 2006:58.
13. Renzo G. Screening and diagnosis of Hemoglobin disorders In: Prevention of Thalassemias and other Hemoglobin disorders volume 1, 2003:58
14. US Department of health and human services/public health service. Brilliant cresyl blue test for inclusion bodies. In:laboratory methods for detecting hemoglobinopathies, Atlanta: centre for disease control, 1984:129-31.
15. Wild BJ, Stephens AD. The use of automated HPLC to detect and quantitative haemoglobins. Clin Lab haematol 1997;19:171-6.
16. Bain BJ. Haemoglobinopathy diagnosis ,oxford,England, Blackwell science Ltd.[2001:260PP].
17. Clarke G, Higgins TN. Laboratory investigation of Haemoglobinopathies and Thalassemia, review and update. Clin Chem 2000,46;128490.
Published
How to Cite
Issue
Section
AUTHORS WHO PUBLISH WITH THIS JOURNAL AGREE TO THE FOLLOWING TERMS:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
.