Acute Toxicity of Ethanolic Extract of Leucaena leucocephala (Lam) de Wit Seeds in Wistar Rats
DOI:
https://doi.org/10.22270/ajprd.v9i1.920Keywords:
L.leucocephala, acute toxicity, ethanolic extractAbstract
Objective: This study was conducted to investigate the toxic effect of ethanol extract of Leucaena leucocephala(Lam) de Wit seeds(EELLS) in rats.
Method: simplicia powder of L.leucochepala seeds extracted by maceration with 80% of ethanol. Evaluation of toxicity effect used the fixed dose method which refer to OECD (Organization of Economic Cooperation and Development)420.
Result: Acute toxicity evaluation in this experiment using different doses varying from 50-2000mg/kgbw, did not result in the death of any animals until 14 days of observation in the experimental evaluation period and there was no change in behavior towards rats.
Conclusion: These results have shown that oral administration of L. leuceocephala did not produce any significant toxic effect in rats. Hence, L. leucocephala could be regarded as a safe natural product for therapeutic use
Downloads
References
2. Loha, M., Mulu, A., Abay, M.S.,Ergete, W., and Geleta, B. Acute and Subacute Toxicity of Methanol Extract of Szygiumguineense Leaves on the Histology of the Liver and Kidney and Biochemical Compositions of Bloods in Rats. Evidance-Based Complementary and Alternative Medicine. 2019; 1-15
3. Utami, A.D., Khairunnisa and Marianne. Study on Acute Oral Toxicity of Ethanolic Extract of Annona squamosa Leaves in Mice (Mus musculus). Indonesian Journal of Pharmaceutical and Clinical Research. 2018; 1(1):56-63
4. Chowtivannakul, P., Srichaikul, B., Talubmook, C. Antidiabetic and antioxidant activities of seed from Leucaena leucocephala (Lam.) de Wit. Agriculture and Natural Resources. 2016; 50:357-361.
5. Zayed, M,Z., AiminWu., Sallam, S. Comparative Phytochemical Constituents of Leucaena leucocephala (Lam.) Leaves, Fruit, Stem Bark and Wood Branches Growin in Egypt using GC-MS Method Coupled with Multivariate Stastical Approaches. BioResources. 2019; 14(1): 996-1013
6. Chanwitheesuk, A., Teerawutgulrag, A., Rakariyatham, N. Screening of Antioxidant Activity and Antioxidant Compounds of Some dible Plants of Thailand. J. Food Chemistry. 2005; 92:491–497.
7. Husein, S., Nainggolan, M., Yuandani and Fanany, I. Evaluation of Antidiarrheal Activity of the Ethanol Extract Leucaena leucocephala (Lam) de Wit Seed. Maced. J. Med. Sci. 2020; 8(A):278-282.
8. Deodhar, U.P., Paradkar, A.R., Purohit, A.P. Preliminary evaluation of Leucaena leucocephala seed gum as a tablet binder. Drug Dev. Ind. Pharm. 1998; 2(4) 6:577-582.
9. Verma PRP, Balkishen R. Studies on disintegrant action of Leucaena leucocephala seed gum in ibuprofen tablet and its mechanism. Journal of Scientific and Industrial Research. 2007; 66:550-557.
10. Hodge, R.E., Minich D.M. Modulation of Metabolic Detoxification Pathways using foods and food -derived component: A Scientific Review with Clinical Application. J. Nutr. Metab. 2015: 1-23
11. Indonesian herbal pharmacopea. 1st ed. Jakarta; Departemen Kesehatan Republik Indonesia 2008. p. 174
12. Iwuanyanwu, K.C.P, Amadi, U., Charles. A.I., and yalugo. Evaluationof Acute and Sub-Chronic Oral Toxicity Study of Baker Cleansers Bitter A Polyherbal Drug on Experimental Rats. EXCLI Journal. 2012; 11 632-640
13. Abid, R and Mahmood, R. Acute and Sub-acute Orl Toxicity of Ethanol Extract of Cassia Fistula Fruit in Male
14. Rats. Avicenna Journal of Phytomedicine. 2018; 9(20):117-125
Published
How to Cite
Issue
Section
AUTHORS WHO PUBLISH WITH THIS JOURNAL AGREE TO THE FOLLOWING TERMS:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
.