PROTEIN BINDING: A STUDY OF INTERACTION BETWEEN DICLOFENAC SODIUM AND BOVINE SERUM ALBUMIN

Authors

  • Aseri Ajay Maharishi arvind College of Pharmacy, Ambabari, Jaipur Rajasthan, India
  • Deepak Duggal B R Nahata College of Pharmacy, Mandsaur, Madhya Pradesh, India
  • Anjali Nayak B R Nahata College of Pharmacy, Mandsaur, Madhya Pradesh, India
  • Jitesh Katewa B R Nahata College of Pharmacy, Mandsaur, Madhya Pradesh, India

Keywords:

Bovine serum Albumin, Diclofenac sodium, Protein binding

Abstract

The study was designed to examine the binding of Diclofenac sodium with bovine serum albumin (BSA) (Ex vivo) at different concentration levels of Bovine serum albumin (BSA) under controlled temperature 370C and phosphate buffer pH7.4 conditions. The protein binding behavior of Diclofenac sodium (sodium[o-(2,6-dichloroanilino)phenyl]acetate) in BSA was investigated by U.V spectroscopy Analysis. Spectroscopic estimations of drug release were made with a constant Diclofenac Sodium concentration while varying the concentration of BSA. The drug protein binding is distinguishable at different levels of BSA (mg) as there was a mark decrease in percentage of Diclofenac Sodium release from 77.5 % (Control) to 71.78%. Comparable results were obtained with different BSA concentrations indicating that albumin is probably the responsible protein which decreases the release of drug. The difference in extent of binding of BSA with similar drug samples were significantly showing the promising effect of BSA over Diclofenac Sodium release when drug dissolution profile was observed.

Downloads

Download data is not yet available.

Published

2013-05-01

How to Cite

Ajay, A., Duggal, D., Nayak, A., & Katewa, J. (2013). PROTEIN BINDING: A STUDY OF INTERACTION BETWEEN DICLOFENAC SODIUM AND BOVINE SERUM ALBUMIN. Asian Journal of Pharmaceutical Research and Development, 1(2), 137–140. Retrieved from https://www.ajprd.com/index.php/journal/article/view/41

Issue

Volume 1st Issue 3rd May-June 2013

Section

Research Articles

AUTHORS WHO PUBLISH WITH THIS JOURNAL AGREE TO THE FOLLOWING TERMS:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).